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stack topographic surface map command  (MetaMorph Inc)
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MetaMorph Inc stack topographic surface map command
VLP stimulation of ileal Ca 2+ waves. ( A ) Ca 2+ waves (total signal in pixels) induced by VLPs and effects on the signal by pretreatment with apyrase, BPTU, the general CaCC inhibitor-Ao1, or the specific TMEM16A inhibitor-Ao1 with exosome controls. ANOVA used for statistical evaluation. n = 3–9. ( B ) Ca 2+ waves (total number of waves) induced by VLPs and effects on the signal by pretreatment with BPTU, CaCC inhibitor-Ao1, or TMEM16A inhibitor-Ao1 with exosome controls. ANOVA used for statistical evaluation. n = 3–9. ( C ) Pseudocolored images of single field of human ileal enteroid expressing GCamP-6s showing areas involved in Ca 2+ waves over 60 minutes. Different colors were used for each Ca 2+ wave. If multiple Ca 2+ waves occurred in the same area, only a single color is shown. ( i ) Irradiated virus. ( ii ) Exosomes. ( iii ) VLPs. ( iv ) VLPs after TMEM16inh-Ao1. Results from a single monolayer are shown using data from <xref ref-type=Figure 9 and ( C ). To generate the pseudocolored images, all pixels from within the areas of interest, representing the extent of Ca 2+ waves, were binarized (value 1), then the MetaMorph Stack Topographic Surface Map command was used to generate the figure, which was colorized using MetaMorph Rainbow lookup tables ( blue , low; green , middle; red , high). Each of the 1800 time points had a grayscale value (0–255) assigned, then the 8-bit color image was converted to 24-bit color for illustration. " width="250" height="auto" />
Stack Topographic Surface Map Command, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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VLP stimulation of ileal Ca 2+ waves. ( A ) Ca 2+ waves (total signal in pixels) induced by VLPs and effects on the signal by pretreatment with apyrase, BPTU, the general CaCC inhibitor-Ao1, or the specific TMEM16A inhibitor-Ao1 with exosome controls. ANOVA used for statistical evaluation. n = 3–9. ( B ) Ca 2+ waves (total number of waves) induced by VLPs and effects on the signal by pretreatment with BPTU, CaCC inhibitor-Ao1, or TMEM16A inhibitor-Ao1 with exosome controls. ANOVA used for statistical evaluation. n = 3–9. ( C ) Pseudocolored images of single field of human ileal enteroid expressing GCamP-6s showing areas involved in Ca 2+ waves over 60 minutes. Different colors were used for each Ca 2+ wave. If multiple Ca 2+ waves occurred in the same area, only a single color is shown. ( i ) Irradiated virus. ( ii ) Exosomes. ( iii ) VLPs. ( iv ) VLPs after TMEM16inh-Ao1. Results from a single monolayer are shown using data from <xref ref-type=Figure 9 and ( C ). To generate the pseudocolored images, all pixels from within the areas of interest, representing the extent of Ca 2+ waves, were binarized (value 1), then the MetaMorph Stack Topographic Surface Map command was used to generate the figure, which was colorized using MetaMorph Rainbow lookup tables ( blue , low; green , middle; red , high). Each of the 1800 time points had a grayscale value (0–255) assigned, then the 8-bit color image was converted to 24-bit color for illustration. " width="100%" height="100%">

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: COVID-19 Diarrhea is Inflammatory, Caused by Direct Viral Effects Plus Major Role of Virus-induced Cytokines

doi: 10.1016/j.jcmgh.2024.101383

Figure Lengend Snippet: VLP stimulation of ileal Ca 2+ waves. ( A ) Ca 2+ waves (total signal in pixels) induced by VLPs and effects on the signal by pretreatment with apyrase, BPTU, the general CaCC inhibitor-Ao1, or the specific TMEM16A inhibitor-Ao1 with exosome controls. ANOVA used for statistical evaluation. n = 3–9. ( B ) Ca 2+ waves (total number of waves) induced by VLPs and effects on the signal by pretreatment with BPTU, CaCC inhibitor-Ao1, or TMEM16A inhibitor-Ao1 with exosome controls. ANOVA used for statistical evaluation. n = 3–9. ( C ) Pseudocolored images of single field of human ileal enteroid expressing GCamP-6s showing areas involved in Ca 2+ waves over 60 minutes. Different colors were used for each Ca 2+ wave. If multiple Ca 2+ waves occurred in the same area, only a single color is shown. ( i ) Irradiated virus. ( ii ) Exosomes. ( iii ) VLPs. ( iv ) VLPs after TMEM16inh-Ao1. Results from a single monolayer are shown using data from Figure 9 and ( C ). To generate the pseudocolored images, all pixels from within the areas of interest, representing the extent of Ca 2+ waves, were binarized (value 1), then the MetaMorph Stack Topographic Surface Map command was used to generate the figure, which was colorized using MetaMorph Rainbow lookup tables ( blue , low; green , middle; red , high). Each of the 1800 time points had a grayscale value (0–255) assigned, then the 8-bit color image was converted to 24-bit color for illustration.

Article Snippet: To generate the pseudocolored images, all pixels from within the areas of interest, representing the extent of Ca 2+ waves, were binarized (value 1), then the MetaMorph Stack Topographic Surface Map command was used to generate the figure, which was colorized using MetaMorph Rainbow lookup tables ( blue , low; green , middle; red , high).

Techniques: Expressing, Irradiation, Virus